O. Reg. 230/08: SPECIES AT RISK IN ONTARIO LIST, Endangered Species Act, 2007
Endangered Species Act, 2007
species at risk in ontario list
Historical version for the period June 5, 2025 to March 29, 2026.
Note: This Regulation is revoked on a day to be named by order of the Lieutenant Governor in Council. (See: 2025, c. 4, Sched. 10, s. 69)
Last amendment: 2025, c. 4, Sched. 10, s. 69.
Legislative History: 56/09, 332/09, 72/10, 373/10, 224/11, 4/12, 25/13, 139/14, 66/15, 200/16, 167/17, 404/18, 24/22, 9/23, 11/24, 16/25, 2025, c. 4, Sched. 10, s. 69.
This is the English version of a bilingual regulation.
Extirpated species
1. The species listed in Columns 3 and 4 of Schedule 1 according to their common and scientific names, and belonging to the species grouping referred to in Column 2 opposite the listed species, are classified by COSSARO as extirpated species. O. Reg. 139/14, s. 1.
Endangered species
2. The species listed in Columns 3 and 4 of Schedule 2 according to their common and scientific names, and belonging to the species grouping referred to in Column 2 opposite the listed species, are classified by COSSARO as endangered species. O. Reg. 139/14, s. 1.
Threatened species
3. The species listed in Columns 3 and 4 of Schedule 3 according to their common and scientific names, and belonging to the species grouping referred to in Column 2 opposite the listed species, are classified by COSSARO as threatened species. O. Reg. 139/14, s. 1.
Special concern species
4. The species listed in Columns 3 and 4 of Schedule 4 according to their common and scientific names, and belonging to the species grouping referred to in Column 2 opposite the listed species, are classified by COSSARO as special concern species. O. Reg. 139/14, s. 1.
Geographical limitations
5. If the classification of a species applies only to a specified geographic area in Ontario, the area is described in a footnote to the relevant Schedule. O. Reg. 230/08, s. 5.
6. Omitted (provides for coming into force of provisions of this Regulation). O. Reg. 230/08, s. 6.
Schedule 1
Extirpated species
| Column 1 | Column 2 | Column 3 | Column 4 |
| 1. | Mosses | Incurved Grizzled Moss | Ptychomitrium incurvum |
| 2. | Vascular Plants | Illinois Tick-trefoil | Desmodium illinoense |
| 3. | Vascular Plants | Spring Blue-eyed Mary | Collinsia verna |
| 4. | Insects | American Burying Beetle | Nicrophorus americanus |
| 5. | Insects | Eastern Persius Duskywing | Erynnis persius persius |
| 6. | Insects | Frosted Elfin | Callophrys irus |
| 7. | Insects | Karner Blue | Plebejus samuelis |
| 8. | Fishes | Gravel Chub | Erimystax x-punctatus |
| 9. | Fishes | Paddlefish | Polyodon spathula |
| 10. | Amphibians | Blanchard’s Cricket Frog | Acris blanchardi |
| 11. | Amphibians | Eastern Tiger Salamander | Ambystoma tigrinum |
| 12. | Revoked: O. Reg. 9/23, s. 1. | ||
| 13. | Reptiles | Eastern Box Turtle | Terrapene carolina |
| 14. | Reptiles | Timber Rattlesnake | Crotalus horridus |
| 15. | Birds | Eskimo Curlew | Numenius borealis |
| 16. | Birds | Greater Prairie-Chicken | Tympanuchus cupido pinnatus |
O. Reg. 200/16, s. 1; O. Reg. 24/22, s. 1; O. Reg. 9/23, s. 1; O. Reg. 11/24, s. 1.
SCHEDULE 2
Endangered species
| Column 1 | Column 2 | Column 3 | Column 4 |
| 0.1 | Lichens | Golden-eye Lichen (Great Lakes population) | Teloschistes chrysophthalmus |
| 1. | Lichens | Pale-bellied Frost Lichen | Physconia subpallida |
| 2. | Lichens | White-rimmed Shingle Lichen | Fuscopannaria leucosticta |
| 3. | Vascular Plants | American Chestnut | Castanea dentata |
| 4. | Vascular Plants | American Columbo | Frasera caroliniensis |
| 5. | Revoked: O. Reg. 11/24, s. 2 (1). | ||
| 6. | Vascular Plants | Bent Spike-rush | Eleocharis geniculata |
| 7. | Vascular Plants | Bird’s-foot Violet | Viola pedata |
| 7.1 | Vascular Plants | Black Ash | Fraxinus nigra |
| 8. | Vascular Plants | Bluehearts | Buchnera americana |
| 9. | Vascular Plants | Blunt-lobed Woodsia | Woodsia obtusa |
| 10. | Vascular Plants | Butternut | Juglans cinerea |
| 11. | Vascular Plants | Cherry Birch | Betula lenta |
| 12. | Vascular Plants | Colicroot | Aletris farinosa |
| 13. | Vascular Plants | Cucumber Tree | Magnolia acuminata |
| 13.1 | Vascular Plants | Downy Yellow False Foxglove | Aureolaria virginica |
| 14. | Vascular Plants | Drooping Trillium | Trillium flexipes |
| 15. | Vascular Plants | Eastern Flowering Dogwood | Cornus florida |
| 16. | Vascular Plants | Eastern Prairie Fringed-orchid | Platanthera leucophaea |
| 17. | Vascular Plants | Eastern Prickly-pear Cactus | Opuntia cespitosa |
| 18. | Vascular Plants | Engelmann’s Quillwort | Isoetes engelmannii |
| 19. | Vascular Plants | False Hop Sedge | Carex lupuliformis |
| 20. | Vascular Plants | Few-flowered Club-rush | Trichophorum planifolium |
| 21. | Vascular Plants | Forked Three-awned Grass | Aristida basiramea |
| 22. | Vascular Plants | Four-leaved Milkweed | Asclepias quadrifolia |
| 23. | Vascular Plants | Gattinger’s Agalinis | Agalinis gattingeri |
| 23.1 | Vascular Plants | Gillman’s Goldenrod | Solidago gillmanii |
| 24. | Vascular Plants | Heart-leaved Plantain | Plantago cordata |
| 25. | Vascular Plants | Hoary Mountain-mint | Pycnanthemum incanum |
| 26. | Vascular Plants | Horsetail Spike-rush | Eleocharis equisetoides |
| 27. | Vascular Plants | Juniper Sedge | Carex juniperorum |
| 28. | Vascular Plants | Large Whorled Pogonia | Isotria verticillata |
| 29. | Vascular Plants | Lowland Toothcup | Rotala ramosior |
| 30. | Vascular Plants | Nodding Pogonia | Triphora trianthophoros |
| 31. | Vascular Plants | Odgen’s Pondweed | Potamogeton x ogdenii |
| 32. | Vascular Plants | Pink Milkwort | Polygala incarnata |
| 32.1 | Vascular Plants | Pumpkin Ash | Fraxinus profunda |
| 33. | Vascular Plants | Red Mulberry | Morus rubra |
| 34. | Vascular Plants | Scarlet Ammannia | Ammannia robusta |
| 35. | Revoked: O. Reg. 24/22, s. 2 (6). | ||
| 36. | Vascular Plants | Skinner’s Agalinis | Agalinis skinneriana |
| 37. | Vascular Plants | Slender Bush-clover | Lespedeza virginica |
| 38. | Vascular Plants | Small White Lady’s-slipper | Cypripedium candidum |
| 39. | Vascular Plants | Small Whorled Pogonia | Isotria medeoloides |
| 40. | Vascular Plants | Stiff-leaved Showy Goldenrod | Solidago rigidiuscula |
| 41. | Vascular Plants | Virginia Goat’s-rue | Tephrosia virginiana |
| 42. | Vascular Plants | Virginia Mallow | Ripariosida hermaphrodita |
| 43. | Revoked: O. Reg. 9/23, s. 2 (1). | ||
| 44. | Vascular Plants | White Prairie Gentian | Gentiana alba |
| 45. | Vascular Plants | Wood-poppy | Stylophorum diphyllum |
| 46. | Molluscs | Broad-banded Forestsnail | Allogona profunda |
| 47. | Molluscs | Eastern Banded Tigersnail | Anguispira kochi kochi |
| 48. | Molluscs | Fawnsfoot | Truncilla donaciformis |
| 49. | Molluscs | Hickorynut | Obovaria olivaria |
| 50. | Molluscs | Kidneyshell | Ptychobranchus fasciolaris |
| 51. | Molluscs | Northern Riffleshell | Epioblasma torulosa rangiana |
| 52. | Molluscs | Proud Globelet | Patera pennsylvanica |
| 53. | Molluscs | Rayed Bean | Villosa fabalis |
| 54. | Molluscs | Round Hickorynut | Obovaria subrotunda |
| 55. | Molluscs | Round Pigtoe | Pleurobema sintoxia |
| 56. | Molluscs | Salamander Mussel | Simpsonaias ambigua |
| 56.1 | Molluscs | Shagreen | Inflectarius inflectus |
| 57. | Molluscs | Snuffbox | Epioblasma triquetra |
| 57.0.1 | Molluscs | Striped Whitelip | Webbhelix multilineata |
| 57.1 | Molluscs | Toothed Globe | Mesodon zaletus |
| 58. | Revoked: O. Reg. 9/23, s. 2 (3). | ||
| 59. | Insects | Bogbean Buckmoth | Hemileuca sp. |
| 59.1 | Insects | False-foxglove Sun Moth | Pyrrhia aurantiago |
| 60. | Insects | Gypsy Cuckoo Bumble Bee | Bombus bohemicus |
| 61. | Insects | Hine’s Emerald | Somatochlora hineana |
| 62. | Insects | Hoptree Borer | Prays atomocella |
| 63. | Insects | Hungerford’s Crawling Water Beetle | Brychius hungerfordi |
| 64. | Insects | Laura’s Clubtail | Stylurus laurae |
| 65. | Insects | Mottled Duskywing | Erynnis martialis |
| 66. | Insects | Nine-spotted Lady Beetle | Coccinella novemnotata |
| 67. | Insects | Northern Barrens Tiger Beetle | Cicindela patruela |
| 68. | Insects | Pygmy Snaketail | Ophiogomphus howei |
| 69. | Revoked: O. Reg. 9/23, s. 2 (3). | ||
| 70. | Insects | Riverine Clubtail | Stylurus amnicola |
| 71. | Insects | Rusty-patched Bumble Bee | Bombus affinis |
| 71.0.1 | Insects | Suckley’s Cuckoo Bumble Bee | Bombus suckleyi |
| 71.1 | Insects | Transverse Lady Beetle | Coccinella transversoguttata |
| 72. | Fishes | American Eel | Anguilla rostrata |
| 73. | Fishes | Eastern Sand Darter (West Lake population) | Ammocrypta pellucida |
| 73.0.1 | Fishes | Lake Chubsucker | Erimyzon sucetta |
| 73.1 | Fishes | Lake Sturgeon (Great Lakes - Upper St. Lawrence populations) | Acipenser fulvescens |
| 74. | Fishes | Northern Madtom | Noturus stigmosus |
| 75. | Fishes | Redside Dace | Clinostomus elongatus |
| 76. | Fishes | River Darter (Great Lakes - Upper St. Lawrence populations) | Percina shumardi |
| 77. | Fishes | Shortnose Cisco | Coregonus reighardi |
| 78. | Fishes | Spotted Gar | Lepisosteus oculatus |
| 79. | Fishes | Warmouth | Lepomis gulosus |
| 80. | Amphibians | Allegheny Mountain Dusky Salamander | Desmognathus ochrophaeus |
| 81. | Amphibians | Fowler’s Toad | Anaxyrus fowleri |
| 82. | Amphibians | Jefferson Salamander | Ambystoma jeffersonianum |
| 83. | Amphibians | Northern Dusky Salamander | Desmognathus fuscus |
| 84. | Amphibians | Small-mouthed Salamander | Ambystoma texanum |
| 85. | Amphibians | Unisexual Ambystoma (Jefferson Salamander dependent population) | Ambystoma laterale – (2) jeffersonianum |
| 86. | Amphibians | Unisexual Ambystoma (Small-mouthed Salamander dependent population) | Ambystoma laterale – texanum |
| 87. | Reptiles | Blue Racer | Coluber constrictor foxii |
| 88. | Reptiles | Butler’s Gartersnake | Thamnophis butleri |
| 89. | Reptiles | Common Five-lined Skink (Carolinian population) | Plestiodon fasciatus |
| 90. | Revoked: O. Reg. 11/24, s. 2 (4). | ||
| 91. | Reptiles | Gray Ratsnake (Carolinian population) | Pantherophis spiloides |
| 92. | Reptiles | Massasauga (Carolinian population) | Sistrurus catenatus |
| 93. | Reptiles | Queensnake | Regina septemvittata |
| 94. | Reptiles | Spiny Softshell | Apalone spinifera |
| 95. | Reptiles | Spotted Turtle | Clemmys guttata |
| 96. | Reptiles | Wood Turtle | Glyptemys insculpta |
| 97. | Birds | Acadian Flycatcher | Empidonax virescens |
| 98. | Birds | Barn Owl | Tyto alba |
| 99. | Birds | Golden Eagle | Aquila chrysaetos |
| 100. | Birds | Henslow’s Sparrow | Ammodramus henslowii |
| 101. | Birds | King Rail | Rallus elegans |
| 102. | Birds | Kirtland’s Warbler | Setophaga kirtlandii |
| 103. | Birds | Loggerhead Shrike | Lanius ludovicianus |
| 104. | Birds | Northern Bobwhite | Colinus virginianus |
| 105. | Birds | Piping Plover | Charadrius melodus |
| 106. | Birds | Prothonotary Warbler | Protonotaria citrea |
| 106.1 | Birds | Red-headed Woodpecker | Melanerpes erythrocephalus |
| 107. | Birds | Red Knot — rufa subspecies (Southeastern USA / Gulf of Mexico / Caribbean wintering population) | Calidris canutus rufa |
| 107.1 | Birds | Red Knot — rufa subspecies (Tierra del Fuego / Patagonia wintering population) | Calidris canutus rufa |
| 108. | Birds | Yellow-breasted Chat | Icteria virens |
| 109. | Mammals | American Badger (Northwestern Ontario population) | Taxidea taxus taxus |
| 110. | Mammals | American Badger (Southwestern Ontario population) | Taxidea taxus jacksoni |
| 110.1 | Mammals | Eastern Red Bat | Lasiurus borealis |
| 111. | Mammals | Eastern Small-footed Myotis | Myotis leibii |
| 111.1 | Mammals | Hoary Bat | Lasiurus cinereus |
| 112. | Mammals | Little Brown Myotis | Myotis lucifugus |
| 113. | Revoked: O. Reg. 11/24, s. 2 (4). | ||
| 114. | Mammals | Northern Myotis | Myotis septentrionalis |
| 114.1 | Mammals | Silver-haired Bat | Lasionycteris noctivagans |
| 115. | Mammals | Tri-colored Bat | Perimyotis subflavus |
O. Reg. 167/17, s. 1; O. Reg. 404/18, s. 1; O. Reg. 24/22, s. 2; O. Reg. 9/23, s. 2; O. Reg. 11/24, s. 2; O. Reg. 16/25, s. 1.
SCHEDULE 3
threatened species
| Column 1 | Column 2 | Column 3 | Column 4 |
| 0.1 | Mosses | Spoon-leaved Moss | Bryoandersonia illecebra |
| 0.2 | Vascular Plants | American Ginseng | Panax quinquefolius |
| 1. | Vascular Plants | American Water-willow | Justicia americana |
| 2. | Vascular Plants | Blue Ash | Fraxinus quadrangulata |
| 3. | Vascular Plants | Branched Bartonia | Bartonia paniculata |
| 4. | Vascular Plants | Deerberry | Vaccinium stamineum |
| 5. | Vascular Plants | Dense Blazing Star | Liatris spicata |
| 6. | Vascular Plants | Dwarf Hackberry | Celtis tenuifolia |
| 7. | Revoked: O. Reg. 11/24, s. 3 (2). | ||
| 8. | Vascular Plants | Fern-leaved Yellow False Foxglove | Aureolaria pedicularia |
| 8.1 | Vascular Plants | Hairy Valerian | Valeriana edulis ssp. ciliata |
| 9. | Vascular Plants | Hill’s Thistle | Cirsium hillii |
| 10. | Vascular Plants | Houghton’s Goldenrod | Solidago houghtonii |
| 11. | Vascular Plants | Kentucky Coffee-tree1 | Gymnocladus dioicus |
| 12. | Revoked: O. Reg. 9/23, s. 3 (3). | ||
| 12.1 | Vascular Plants | Pale Showy Goldenrod | Solidago pallida |
| 13. | Vascular Plants | Pitcher’s Thistle | Cirsium pitcheri |
| 14. | Vascular Plants | Purple Twayblade | Liparis liliifolia |
| 15. | Vascular Plants | Round-leaved Greenbrier | Smilax rotundifolia |
| 16. | Revoked: O. Reg. 24/22, s. 3 (4). | ||
| 17. | Vascular Plants | Small-flowered Lipocarpha | Cyperus subsquarrosus |
| 17.0.1 | Vascular Plants | Smooth Yellow False Foxglove | Aureolaria flava |
| 17.1 | Vascular Plants | Spotted Wintergreen | Chimaphila maculata |
| 17.2 | Vascular Plants | Western Silvery Aster | Symphyotrichum sericeum |
| 18. | Vascular Plants | White Wood Aster | Eurybia divaricata |
| 19. | Vascular Plants | Wild Hyacinth | Camassia scilloides |
| 20. | Vascular Plants | Willowleaf Aster | Symphyotrichum praealtum |
| 20.1 | Molluscs | Carolina Mantleslug | Philomycus carolinianus |
| 21. | Molluscs | Lilliput | Toxolasma parvum |
| 22. | Molluscs | Purple Wartyback | Cyclonaias tuberculata |
| 23. | Molluscs | Threehorn Wartyback | Obliquaria reflexa |
| 24. | Molluscs | Wavy-rayed Lampmussel | Lampsilis fasciola |
| 24.1 | Insects | Davis’s Shieldback | Atlanticus davisi |
| 25. | Insects | Lake Huron Grasshopper | Trimerotropis huroniana |
| 25.0.1 | Insects | Northern Oak Hairstreak | Satyrium favonius ontario |
| 25.1 | Insects | Rapids Clubtail | Gomphus quadricolor |
| 25.2 | Insects | Reversed Haploa Moth | Haploa reversa |
| 25.3 | Insects | Skillet Clubtail | Gomphurus ventricosus |
| 26. | Fishes | Black Redhorse | Moxostoma duquesnei |
| 27. | Revoked: O. Reg. 404/18, s. 2 (5). | ||
| 28. | Fishes | Cutlip Minnow | Exoglossum maxillingua |
| 29. | Fishes | Eastern Sand Darter (Southwestern Ontario population) | Ammocrypta pellucida |
| 30. | Revoked: O. Reg. 404/18, s. 2 (5). | ||
| 31. | Fishes | Lake Sturgeon (Saskatchewan - Nelson River populations) | Acipenser fulvescens |
| 31.1 | Fishes | Lake Whitefish (Opeongo Lake large-bodied populations) | Coregonus clupeaformis |
| 31.2 | Fishes | Lake Whitefish (Opeongo Lake small-bodied populations) | Coregonus clupeaformis |
| 32. | Fishes | Pugnose Minnow | Opsopoeodus emiliae |
| 33. | Fishes | Pugnose Shiner | Notropis anogenus |
| 34. | Fishes | Shortjaw Cisco | Coregonus zenithicus |
| 35. | Fishes | Silver Chub | Macrhybopsis storeriana |
| 36. | Fishes | Silver Shiner | Notropis photogenis |
| 37. | Reptiles | Blanding’s Turtle | Emydoidea blandingii |
| 37.1 | Reptiles | Eastern Foxsnake (Carolinian population) | Pantherophis vulpinus |
| 38. | Reptiles | Eastern Foxsnake (Great Lakes / St. Lawrence population) | Pantherophis vulpinus |
| 39. | Reptiles | Eastern Hog-nosed Snake | Heterodon platirhinos |
| 40. | Reptiles | Gray Ratsnake (Frontenac Axis population) | Pantherophis spiloides |
| 41. | Reptiles | Massasauga (Great Lakes - St. Lawrence population) | Sistrurus catenatus |
| 42. | Birds | American White Pelican | Pelecanus erythrorhynchos |
| 43. | Birds | Bank Swallow | Riparia riparia |
| 44. | Revoked: O. Reg. 9/23, s. 3 (5). | ||
| 45. | Birds | Bobolink | Dolichonyx oryzivorus |
| 46. | Birds | Cerulean Warbler | Setophaga cerulea |
| 47. | Birds | Chimney Swift | Chaetura pelagica |
| 48. | Birds | Eastern Meadowlark | Sturnella magna |
| 49. | Revoked: O. Reg. 16/25, s. 2. | ||
| 49.1 | Birds | Hudsonian Godwit | Limosa haemastica |
| 50. | Birds | Least Bittern | Ixobrychus exilis |
| 50.1 | Birds | Lesser Yellowlegs | Tringa flavipes |
| 51. | Birds | Louisiana Waterthrush | Parkesia motacilla |
| 52. | Birds | Short-eared Owl | Asio flammeus |
| 53. | Mammals | Caribou (Boreal population) | Rangifer tarandus |
| 53.1 | Mammals | Eastern Wolf | Canis sp. cf. lycaon |
| 54. | Mammals | Gray Fox | Urocyon cinereoargenteus |
| 55. | Mammals | Polar Bear | Ursus maritimus |
| 56. | Mammals | Wolverine | Gulo gulo |
Note:
1 The species set out in Item 11 of this Schedule is classified by COSSARO as a threatened species but the classification applies only to the following geographic areas: the County of Elgin, the County of Essex, the County of Lambton, the County of Middlesex, the County of Norfolk, the County of Oxford and the Municipality of Chatham-Kent.
O. Reg. 167/17, s. 1; O. Reg. 404/18, s. 2; O. Reg. 24/22, s. 3; O. Reg. 9/23, s. 3; O. Reg. 11/24, s. 3; O. Reg. 16/25, s. 2.
SCHEDULE 4
special concern species
| Column 1 | Column 2 | Column 3 | Column 4 |
| 0.1 | Vascular Plants | American Hart’s Tongue Fern | Asplenium scolopendrium var. americanum |
| 1. | Vascular Plants | Broad Beech Fern | Phegopteris hexagonoptera |
| 2. | Vascular Plants | Climbing Prairie Rose | Rosa setigera |
| 3. | Vascular Plants | Common Hoptree | Ptelea trifoliata |
| 4. | Vascular Plants | Crooked-stem Aster | Symphyotrichum prenanthoides |
| 5. | Vascular Plants | Dwarf Lake Iris | Iris lacustris |
| 5.0.1 | Vascular Plants | Eastern False Rue-anemone | Enemion biternatum |
| 5.1 | Vascular Plants | Goldenseal | Hydrastis canadensis |
| 6. | Vascular Plants | Green Dragon | Arisaema dracontium |
| 7. | Revoked: O. Reg. 404/18, s. 3 (2). | ||
| 8. | Vascular Plants | Hill’s Pondweed | Potamogeton hillii |
| 8.1 | Vascular Plants | Lakeside Daisy | Tetraneuris herbacea |
| 9. | Vascular Plants | Riddell’s Goldenrod | Solidago riddellii |
| 10. | Vascular Plants | Shumard Oak | Quercus shumardii |
| 11. | Vascular Plants | Swamp Rose-mallow | Hibiscus moscheutos |
| 12. | Vascular Plants | Tuberous Indian-plantain | Arnoglossum plantagineum |
| 12.1 | Molluscs | Eastern Pondmussel | Ligumia nasuta |
| 12.2 | Molluscs | Mapleleaf | Quadrula quadrula |
| 13. | Molluscs | Rainbow | Villosa iris |
| 13.1 | Insects | American Bumble Bee | Bombus pensylvanicus |
| 13.2 | Insects | Dukes’ Skipper | Euphyes dukesi |
| 14. | Insects | Monarch | Danaus plexippus |
| 14.1 | Insects | Red-tailed Leafhopper | Aflexia rubranura |
| 15. | Insects | West Virginia White | Pieris virginiensis |
| 16. | Insects | Yellow-banded Bumble Bee | Bombus terricola |
| 17. | Fishes | Blackstripe Topminnow | Fundulus notatus |
| 18. | Fishes | Bridle Shiner | Notropis bifrenatus |
| 18.1 | Fishes | Channel Darter | Percina copelandi |
| 19. | Fishes | Grass Pickerel | Esox americanus vermiculatus |
| 20. | Fishes | Lake Sturgeon (Southern Hudson Bay - James Bay populations) | Acipenser fulvescens |
| 21. | Fishes | Northern Brook Lamprey | Ichthyomyzon fossor |
| 22. | Fishes | Northern Sunfish (Great Lakes - Upper St. Lawrence populations) | Lepomis peltastes |
| 23. | Fishes | River Redhorse | Moxostoma carinatum |
| 24. | Fishes | Silver Lamprey (Great Lakes - Upper St. Lawrence River population) | Ichthyomyzon unicuspis |
| 25. | Fishes | Spotted Sucker | Minytrema melanops |
| 26. | Fishes | Upper Great Lakes Kiyi | Coregonus kiyi kiyi |
| 27. | Reptiles | Common Five-lined Skink (Southern Shield population) | Plestiodon fasciatus |
| 28. | Reptiles | Eastern Musk Turtle | Sternotherus odoratus |
| 29. | Reptiles | Eastern Ribbonsnake | Thamnophis sauritus |
| 30. | Reptiles | Lake Erie Watersnake | Nerodia sipedon insularum |
| 31. | Reptiles | Northern Map Turtle | Graptemys geographica |
| 32. | Reptiles | Snapping Turtle | Chelydra serpentina |
| 33. | Revoked: O. Reg. 11/24, s. 4 (2). | ||
| 33.1 | Birds | Barn Swallow | Hirundo rustica |
| 34. | Birds | Black Tern | Chlidonias niger |
| 35. | Birds | Canada Warbler | Cardellina canadensis |
| 36. | Birds | Common Nighthawk | Chordeiles minor |
| 36.1 | Birds | Eastern Whip-poor-will | Antrostomus vociferus |
| 37. | Birds | Eastern Wood-Pewee | Contopus virens |
| 37.1 | Birds | Evening Grosbeak | Coccothraustes vespertinus |
| 38. | Birds | Golden-winged Warbler | Vermivora chrysoptera |
| 39. | Birds | Grasshopper Sparrow | Ammodramus savannarum |
| 40. | Birds | Horned Grebe | Podiceps auritus |
| 41. | Birds | Olive-sided Flycatcher | Contopus cooperi |
| 42. | Birds | Peregrine Falcon | Falco peregrinus |
| 43. | Birds | Red Knot — rufa subspecies (Northeastern South America wintering population) | Calidris canutus rufa |
| 44. | Birds | Red-necked Phalarope | Phalaropus lobatus |
| 44.1 | Birds | Rusty Blackbird | Euphagus carolinus |
| 45. | Revoked: O. Reg. 9/23, s. 4 (2). | ||
| 46. | Birds | Wood Thrush | Hylocichla mustelina |
| 47. | Birds | Yellow Rail | Coturnicops noveboracensis |
| 48. | Revoked: O. Reg. 9/23, s. 4 (2). | ||
| 48.1 | Mammals | Caribou (Eastern Migratory population) | Rangifer tarandus |
| 48.2 | Mammals | Cougar | Puma concolor |
| 49. | Mammals | Eastern Mole | Scalopus aquaticus |
| 50. | Mammals | Woodland Vole | Microtus pinetorum |
O. Reg. 167/17, s. 1; O. Reg. 404/18, s. 3; O. Reg. 24/22, s. 4; O. Reg. 9/23, s. 4; O. Reg. 11/24, s. 4; O. Reg. 16/25, s. 3.